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To the preparation out of WPS, the new dehydrated mycelia otherwise dried grown sclerotium was removed 3 x having h2o by sonication to have 29 minute to discover the WPS small fraction. The new particles once WPS removal are removed 30 minutes having step one M NaOH service under sonication to have 31 min to find the APS fraction. The newest WPS and you will APS options had been then modified to 80% ethanol, as well as the comes down were accumulated and you may dried as mentioned a lot more than. Depending on the NIST spectral collection, along with 24 alcohols, six aldehydes, eleven esters, six alkenes, and you may 15 almost every other parts, while the shown inside the Dining table 2. The newest part of complete recognized compounds is actually 99.96% and you may 98.03% to have natural oils of culture media and you will a outside level, respectively.

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